十年前,麦克(克里斯•波特 Chris Potter 饰)和他的搭档黑猩猩明奇侦探界的明星搭档,他们屡破奇案。但一次任务的失利却使得麦克心灰意冷,于是他退出了侦探界,专心照顾他的女儿阿梅利亚;而明奇也加入了马戏团开始了它的新生活。转眼间,阿梅利亚(艾玛•罗伯茨 Emma Roberts 饰)12岁了,凭借聪明的头脑她改进的钻孔机获得了国家科学奖,更获得了法尔里博士(理查德•坎德 Richard Kind 饰)邀请前往日本参加研讨会。然而法尔里博士其实另有阴谋,麦克和明奇这对老搭档必须再战江湖,救出身陷囫囵的阿梅利亚!Minkey, a super-spy primate, and his partner Mike were the two best spies in the business. For years, they made every mission possible... But when Mike retired to raise his baby girl, Amelia, he never predicted that life would come full circle. Ten years later, Amelia -- who has invented a revolutionary chemical drill -- is kidnapped and whisked off to Japan by her hero, Dr. Farley, who is planning a highly dangerous experiment that could cause a massive earthquake and destroy the entire world. Mike and Minkey partner up one last time for their most important mission ever: saving Amelia -- and the world -- from the clutches of the evil Dr. Farley.